The 2011-2014 Edition of the Sigma-Aldrich Cell Culture Manual This manual has a variety of valuable cell culture information that has become a must-have in any cell culture lab! Reformatted to be even more useful to researchers, this publication has more detailed content to further help you achieve success in your cell culture work. • More protocols • More Technical Information • More Quick-reference Tables • More Great Products for Cell Culture. Table of Contents • Cell Culture Essentials • Serum-Free and Specialty Media • Classical Media • Sera • Balanced Salt Mixtures • ECACC ® Cell Lines • Labware. Contains in-depth, practical information in the following areas: Media selection Cell Line selection Sterility and Sterile Technique Endotoxin Contamination Mycoplasma Contamination and Clean-up Sera Selection Production Protease/Phosphatase Inhibitors A collection of cell-based kits ranging in application from cytotoxicity and viability to organelle isolation and purification Antibiotic Selection Cytokine/Growth Factor Selection Stem Cell Culture Mouse Embryo Culture Attachment Factor Selection Hybridoma and Recombinant Proteins Cell Dissociation.

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